CAPEL-serisi Capillary Elektroforez Cihazları

METHOD

High performance capillary electrophoresis 

PRINCIPLE OF OPERATION

Based on the differential migration of componenys of aqueous samples within a narrow quartz capillary under the influence of the applied electric field. Separated solutes are quantitatively detected at the capillary outlet by high sensitive optical system based on direct or indirect UV absorbance.

FEATURES AND BENEFITS

• high separation efficiency
• low reagent consumption
• simple sample preparation
• compact benchtop design
• autosamplers (10 inlet and 10 outlet vials) in CAPEL-105 and CAPEL-105M
• user-friendly software
• implementation of various methods (capillary zone electrophoresis, micellar electrokinetic chromatography, capillary isoelectric focusing, capillary gel electrophoresis, capillary isotachophoresis

     New features of Capel-105M:

• full control of the instrument from the PC
• broadened range of pre-set temperatures for liquid cooling
• fully automated programmable system with different levels of task complexity - from manual operation through simple analysis to complex sequence of runs
• extended operation options such as spectra scanning and broader injection pressure range
• ability to apply controlled pressure simultaneously with the high voltage during analysis
• substantially modified capillary cassette to provide liquid cooling, simplify capillary replacement and increase the sensitivity

DESCRIPTION - Automatic pressure or electrokinetic sample injection - Built-in capillary rinsing system - Convenient access to the vials and capillary - Possibility of visual check of the position of the vials and capillary rinsing - Liquid cooling system in CAPEL103 and in CAPEL-105, CAPEL-105M - Built-in monochromator (190 - 380 nm) in CAPEL-105 and CAPEL-105M

Common features for all CAPEL instruments provide clear benefits for their users:

Feature	Benefit
Copmplete control of the instrument from a PC	User friendliness
Highly efficient capillary liquid cooling	Extended range of applied buffers, increased efficiency in separation
Powerful software package "Elforun®"	Increased flexibility in performing analyses of various complexit Any kind of complex runs are possible including those with pre-programming of changes in analysis conditions Customized report, data export to other programs
Extended instrumental options	Spectra scanning facilitates peak identification (only for CAPEL-105M) Broad range of controlled pressure injection permits analysis of viscous samples
Substantially modified capillary cassette	Easy capillary change just in a few minutes Lower Detection Limit due to the optimized optical scheme

Spectrophotometric detector, placed on Capel-105M, enables an operator not only to select the most appropriate wavelength within the UV range (190 - 380 nm) but also to perform spectral scanning of separated compounds, thus supporting in peak identification.

APPLICATION EXAMPLES

Capel®-series CE systems can be supplied with validated measurement procedures (in accordance with EPA, ASTM and other standards).

Environmental analysis

• Soils (mobile forms of Co, Cu, Ni, Zn)
• Drinking, natural and waste water (Br -, I - and other inorganic and organic ions)

Quality control of foodstuffs and beverages

• Mineral and bottled water (inorganic cations and anions)
• Beverages and juices (inorganic cations and anions, sweeteners, antioxidants, vitamins, carbohydrates)
• Wines, brandies and vodkas (inorganic cations and anions, aromatic aldehydes, phenolcarbonyc acids, amino acids)
• Beer (inorganic cations and anions, hop and beer bitter acids (humulones and isohumulones, amino acids, vitamins)
• Tea, coffee (caffeine, catechin)
• Foodstuff (amino acids, synthetic dyes , organic acids, amines, proteins)

Pharmacology

• Technological monitoring and patent medicines analysis
• Enantiomers separation

Biochemistry

• Determination of inorganic cations and anions, amino acids and proteins in biological fluids
• Pharmacokinetics studies
• Protein separation

Forensic studies

• Analysis of explosives and trace detection
• Drugs analysis

Chemical industry

• Technological monitoring
• Composition determination of raw material and intermediate products

DESIGN AND OPERATION FEATURES

Detectors
CAPEL-103 with one wavelength UV-detector lets you detect at 254 nm. CAPEL-105M and CAPEL-105M is equipped with variable wavelength UV-detector, wavelength range 190 - 380 nm.

Vials
Standard 1.5 ml, minimal sample volume 100 µl.

Injection modes
Controlled pressure profile injection with variable peak pressure, programmable peak pressure range being 0 - 60 mbar
Programmable injection time

Electrophoresis power
Voltage range settable from 1 to 25 kV
(manually reversible polarity)
Current from 0 to 200 mA

System control
Membrane keyboard
4 string 20 characters and 30 string 16 characters LCDs in Capel-103 and Capel-105/105M, respectively
Data acquisition and processing system based on a standard PC and Chrom&Spec® software with MSWindows® user interface.

Chrom&Spec® software features
Real time electrophoregram visualization
Electrophoregram data processing
Computation of electrophoresis system parameters
Customized report output (hard copy and file), data exchanges with worksheets, databases and word processors
Extensive data processing algorithm customization
GLP and GALP compliance.
Data exchanges with worksheets, databases and word processors.

EQUIPMENT AND OPTIONS

• capillary electrophoresis system CAPEL®
  (103R, 103RT , 104, 104M, 105, or 105M model)
• spare capillary cassette
• software package (for 103R, 103RT , 104, and 105 models - Chrom@Spec, for 104M and 105M models - Elforun®)
• kits for analysis [on request] ; the CE-kits of any manufacturers are compatible with Capel®-series CE systems

SPECIFICATIONS

CAPEL-103R CAPEL-103RT CAPEL-104Ò CAPEL-104M CAPEL-105 CAPEL-105M Detector 254 nm 190 - 380 nm Analysis Constant voltage 1 - 25 kV in 1-kV steps, manual polarity switching, current 0 - 200 µA Injection By voltage or by pressure Rinsing By pressure 1000 mbar Capillary Length/Internal diameter: 35- 100 cm/75 µm (optional: 50 µm) Capillary cooling Liquid cooling Liquid cooling with thermostabilization, from -10 up to +30oC with respect to ambient temperature Sampler Manual operation Autosamplers for 10 inlet and 10 outlet vials Controlled parameters Time of analysis Pressure Voltage Time of analysis Pressure Temperature Voltage Time of analysis Wavelength Pressure Temperature Voltage Power requirements 110/220 Vac, 50/60 Hz (optional: 85 - 264 Vac) Power consumption 80 W 150 W 200 W Size 420x330x360 mm 420x350x360 mm 500x500x500 mm Weight 16 kg 25 kg 30 (105M) kg

 

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